Effect of resisted training on survival of c57bl / 6 mice with cachexia associated with cutaneous melanoma

Abstract

OBJECTIVE: The objective of this study was to verify the effect of resistance training on the survival of C57BL / 6 mice with cachexia associated with cutaneous melanoma. MATERIAL AND METHODS: The sample consisted of 64 (C57BL / 6 female mice, aged between 10 and 12 weeks, with about 50 ± 5 grams of body weight. The mice were randomly assigned to four groups: i. Control mice, with tumor induction, physically inactive (Control, n = 16); ii. mice undergoing daily resistance training only before tumor induction (Training 1, n = 16); iii. mice undergoing daily resistance training before and after induction tumor (Training 2, n = 16); iv. mice subjected to daily resistance training after tumor induction and presented a caquetic condition (Training 3, n = 16). The shock resistance training (TR) procedure was used with a ladder with 110 cm high, 18 cm wide, 2 cm between the steps and 80 degrees of inclination The procedure for TR with shock was a ladder 110 cm high, 18 cm wide, 2 cm between the steps and 80 degrees of inclination. resisted is based on the rise of the mice, on the exit platform an electric shock was applied as a stimulus to climb the stairs, in this stage the shock was applied to the four legs of the animal with an electrical voltage of 20 volts at a frequency of 45 Hertz during six sets of eight repetitions, each with a ninety second interval between sets The groups of mice were followed up for a maximum of 15 days after the diagnosis of cachexia in order to compare the overall cancer-related survival between the study groups . The Kaplan-Meier survival curves were estimated for each event and the curves of the different groups were compared using the Log-rank test. The proposed survival time was 25 days after inoculation. RESULTS: The results presented in this study showed that there was no significant difference (p <0.05) between the training proposals. CONCLUSION: There was no difference in the survival of animals with cachexia associated with the syngeneic tumor model of cutaneous melanoma with resistance or sedentary exercise intervention.