Avaliação histomorfométrica de feridas cutâneas de ratos submetidas a terapias biomoduladoras – protocolo de pesquisa
DOI:
https://doi.org/10.17267/2675-021Xevidence.2023.e4105Palavras-chave:
Cicatrização de feridas, Terapia de luz de baixo nível, Ozônio, Ratos WistarResumo
INTRODUÇÃO: A utilização de terapias biomoduladoras no intuito de auxiliar o reparo tecidual tem sido cada vez mais comum nas diversas áreas de saúde. OBJETIVO: Este estudo objetiva avaliar, comparativamente, os efeitos da fotobiomodulação laser de 660 nm, ozonioterapia e óleo ozonizado sobre o reparo através da análise histomorfométrica em feridas cutâneas de ratos. Serão utilizados 40 ratos Wistar distribuídos em 4 grupos de 10 animais cada, Grupo controle (GC), Laser (GL), Gás Ozônio (GGO) e Óleo Ozonizado (GOO). MATERIAIS E MÉTODOS: Serão realizados ferimentos cutâneos padronizados no dorso dos animais e os diferentes grupos experimentais serão tratados com as terapias biomoduladoras descritas, por três dias consecutivos. Cinco e 10 dias após a realização da cirurgia, 5 ratos de cada grupo serão eutanasiados. Serão removidos fragmentos de pele, incluindo a área da ferida, para processamento histológico e posterior coloração das secções histológicas com Hematoxilina-eosina e Sírius vermelho. Serão obtidas micrografias das secções histológicas e dez imagens padrão serão capturadas para avaliação quantitativa das variáveis: área de colágeno, número de vasos sanguíneos e espessura do epitélio. As variáveis infiltrado de células inflamatórias polimorfonucleares e monomorfonucleares, assim como edema, serão analisadas semiquantitativamente. Será realizada análise estatística das variáveis do estudo, com nível de significância p<0,05. CONCLUSÃO: Espera-se verificar qual das terapias biomoduladoras utilizadas pode favorecer a resolução do reparo tecidual, em especial, por promover a biossíntese do colágeno.
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Copyright (c) 2023 Flávia Quadros Lima, Antônio Márcio Marchionni, Alena Peixoto Medrado
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The authors retain copyrights, transferring to the Journal of Evidence-Based Healthcare only the right of first publication. This work is licensed under a Creative Commons Attribution 4.0 International License.
