NEURODYNAMIC MOBILIZATION REDUCES INTRANEURAL FIBROSIS AFTER SCIATIC CRUSH LESION IN RATS

Autores

  • Êmyle Martins Lima Federal University of Bahia
  • Herman Henrique Silva Santana Federal University of Bahia
  • Alena Peixoto Medrado Federal University of Bahia
  • Ana Maria Blanco Martinez Federal University of Bahia
  • Abrahão Fontes Baptista Federal University of Bahia

DOI:

https://doi.org/10.17267/2317-3386bjmhh.v5i2.1303

Palavras-chave:

peripheral nerves, nerve regeneration, mast cells, stress, mechanical, collagen

Resumo

Peripheral nerve lesions may be associated with abnormal scarring that lead to regenerative failure and dysfunction. Neurodynamic mobilization (NM) imposes controlled mechanical loads on the peripheral nerve and may influence inflammation and collagen deposition after a lesion. However, there is lack of experimental data to support these claims. Objective: To evaluate the impact of NM in the intraneural number of mast cells, collagen deposition and number of blood vessels after an ischiatic crush lesion in rats. Methods: This is a laboratory animal study, where 20 rats (Rattus norvegicus) were randomly divided into two groups, NM (n=10) and control (n=10), submitted to a right ischiatic nerve lesion. A tensioning NM began 10 days after lesion, and was maintained once a day, six times a week, for three weeks. After this period, the animals were euthanized and the nerves assessed for the number of mast cells, collagen area and number of blood vessels. Results: NM led to a lower number of degranulated mast cells (Kruskal-Wallis=0.29 p<0.05), organization of collagen deposition (Kruskal-Wallis = 0.01, p<0.05 ).  There was no influence of NM on the number of intraneural blood vessels (Kruskal-Wallis=0.46 p<0.05). Conclusion: NM started 10 days after a ischiatic nerve crush lesion modulates the inflammatory process and prevents random deposition of collagen at the lesion site, but has no influence on blood vessels formation.

Biografia do Autor

  • Êmyle Martins Lima, Federal University of Bahia
    MSc in Medicine and Health.Federal University of Bahia, Salvador, Bahia, Brazil.
  • Herman Henrique Silva Santana, Federal University of Bahia
    MSc in Medicine and Health.Federal University of Bahia, Salvador, Bahia, Brazil.
  • Alena Peixoto Medrado, Federal University of Bahia
    Phd in Human Pathology. Adjunct Professor at UFBA- Federal University of Bahia, Salvador, BA, Brazil
  • Ana Maria Blanco Martinez, Federal University of Bahia
    PhD in Neurosciences. Title professor at UFRJ- Federal University of Rio de Janeiro. Rio de Janeiro,Brazil.
  • Abrahão Fontes Baptista, Federal University of Bahia
    PhD in Morphological Sciences. Adjunct Professor at UFBA- Federal University of Bahia, Salvador, BA, Brazil

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Publicado

2017-06-22

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